6 Split-Thickness Skin Grafting
James F. Thornton and Jourdan A. Carboy
Sum m ary
This chapter discusses split-thickness skin grafting for post
Mohs reconstruction. Indications, including detailed intraope-
rative use of the dermatome in skin graft harvest, as well as
considerations for donor-site care are discussed. Particular
attention is directed to the selection of appropriate donor sites
for split-thickness skin grafts.
Keywords: split-thickness skin grafting, dermal regeneration
template, Zimmer dermatome, skin graft harvest site, bolster
Summary
● Split-thickness skin grafting can provide large volumes of
color-matched skin for head and neck reconstruction with
minimal donor-site morbidity.
● Split-thickness skin graft harvesting from the scalp provides
thick color-matched skin with the advantage of the donor
site remaining in the operative field.
● There is little role for meshing split-thickness skin grafts in
head and neck reconstruction given the final aesthetic result
is unacceptable.
6.1 General Considerations
The usefulness of split-thickness skin grafting (STSG) in head
and neck reconstruction may well be more than generally
appreciated and is greatly expanded by STSG in conjunction
with dermal regeneration templates.1 The ease and reliability of
split-thickness grafting and the ability to provide large volumes
of thin color-matched skin that will reliably heal to an excellent
cosmetic result with very acceptable donor-site appearance
makes it a very valuable technique for head and neck recon-
struction.2 The decision to use either a split-thickness color-
matched skin graft or a full-thickness color-matched skin graft
for head and neck reconstruction depends on the quality of the
skin, the size requirements of the skin, as well as considerations
regarding both harvest and donor-site management.
Historically, many surgeons feel that general anesthetic is
required for safe harvest of STSG; however, in our practice at
the UTWSMC, we have comfortably and easily performed STSG
under both local and intravenous sedation. Limiting the use of
anesthesia is safer for elderly patients who may not be suitable
candidates for general anesthesia.
6.2 Donor-Site Selection
The selection of donor site begins with the determination of
whether color-matched skin is required and following the same
principles as for full-thickness skin grafting, if color-matched
skin is required then the graft must be harvested above the
clavicles. In many ways, the scalp is an ideal donor site for
STSG.3,4 It is very thick, very vascular, and provides a robust
graft and if harvested appropriately on shaved hair-bearing
scalp, the donor site heals very rapidly and can heal with a
nearly imperceptible scar.3,4,5 Contrast this versus the very
notable scar that is left following harvest of STSG from the
traditional site on the leg.
For defects that do not require color-matched skin, the lateral
or proximal thigh donor site is very accessible, very easy to
harvest from, and is able to provide large volumes of smooth,
non–color-matched skin; however, it can leave an unsightly
scar, particularly on young females, that is di cult to cover.6
It is preferable to have the patient wear an appropriate under-
wear or swimsuit bottom and then mark out the borders of the
clothing and then take the graft within the borders. For current
fashions, this usually results in a graft that is harvested medially
from a single buttock. Although this will result in painful
donor-site sitting for 5 to 10 days, it does result ultimately in a
scar that can be completely hidden by clothing.6
6.3 Harvest Technique
After selection of the skin graft harvest site, careful measure-
ment of the required graft dimensions and careful infiltration of
the donor site are performed with local anesthetic to provide
hemostasis, pain control, and some degree of turgor to the skin
that will aid in the graft harvest. The selection of the Zimmer
dermatome blade guard is also important, given that oftentimes
the 3-in. guard is the only template that will harvest the entire
dimensions of the guard. A 4-in. guard, although it will harvest
larger than 3 in., will result in the harvested skin not fitting on
the standard meshing carriers. This is also true of the 2-in.
guard that will also oftentimes harvest less than the 2-in. guard
dimensions. The decision on harvested skin graft thickness
involves three factors: (1) the thicker the harvested graft, the
longer the donor-site healing—and with elderly thin skinned
patients, it is possible to inadvertently harvest skin thick
enough to result in a full-thickness donor-site defect; (2) the
thicker the graft, the more prolonged the graft healing; and
(3) thicker grafts heal with less primary and secondary skin
contracture. Additionally, even though the manufacturer rec-
ommends for Integra graft of 0.008" thickness, our clinical
experience with Integra is that a robust Integra base is able to
heal much thicker skin grafts, even up to 0.012" thick, which is
more easily harvested and easier to handle during inset.7
Several considerations are to be made in setting up a derma-
tome for graft harvest. This includes the selection of the blade
guard and ensuring the blade has not been inadvertently placed
upside down. Even though there are safeguards in place on the
dermatome itself to prevent incorrect blade placement, it is still
a possible mistake and will result in the blade guard being inef-
fective, resulting in an instantaneous full-thickness skin defect
when graft harvest is attempted. Care must be taken that a
Padgett blade is also not placed into a Zimmer dermatome,
because this will result in no protection by the guard and a full-
thickness injury to the donor site. In setting up the donor site
after infiltration of local anesthetic, care must be taken that the
Split-Thickness Skin Grafting
33
drapes do not interfere with the full path of the harvest and is
useful to perform a practice pass without power on the derma-
tome to ensure there are no obstructions to harvesting. A very
common practice of using a no. 15 blade as a “feeler gauge” to
measure the blade thickness is infeasible, is not accurate, and is
not recommended by the Zimmer dermatome manuals. This is
a habit that is easily discarded with no ill e ect for the patient.
Mineral oil is routinely used on the guard, as well as on the
donor site. After the graft is harvested, initial attention is to be
focused on the donor site. There is literature in support of an
inclusive dressing of either Tegaderm or BioDerm to be placed
directly over the donor site and great care is taken for the accu-
rate placement of this dressing with wide borders and use of a
tissue adhesive on the normal skin borders to maintain this
dressing in place.8 The patient is given postoperative instruc-
tions to leave the adhesive in place for 5 to 7 days and healing
can occur underneath. If the patient develops a large area of
ballotable fluid unexpectedly, we encourage them to make a
small piercing on the Tegaderm with nail scissors and drain it
and make every attempt to leave the occlusive Tegaderm dress-
ing in place until the donor site heals underneath. For surgeons
who are interested in the final aesthetic outcome, some degree
of insight needs to go into the decision-making process of
actually meshing the graft. Although meshing does increase the
volume of usable skin, and also likely increases the graft take, it
will result in an irretrievably abnormal aesthetic result that is
impossible to correct. The common pebble-stoning or screen
door appearance of a meshed skin graft is unmistakable and is
caused by the healing of the meshed interstices by scarring and
not actual primary healing and results with a far inferior result
than a unmeshed sheet graft. Additionally, the practice of
meshing, but not expanding, also results in wound healing that
is inferior to an appropriately inset unmeshed sheet graft.
6.4 Graft Inset
The inset process for large sheet graft relies on continuous fast
or plain gut sutures (never chromic gut) along the border of the
graft and then dependent on the size, as well as the eventual
contours, small areas of the graft, where it is not adhered or
where there are clear air bubbles underneath which are then
sharply incised with a no. 69 Beaver blade and a 5–0 gut suture
is utilized to secure one limb of the interstices to keep this open
and allow the easy egress of any potential seroma fluid. The
graft is then carefully bolstered with a surgical sponge bolster
with ointment on one side and no intervening nonstick gauze.
For large areas of graft, central 3–0 double-armed Prolene
sutures are utilized to improve uniform compression of the
sponge and the remainder of the sponge is sewn in place with
4–0 pop-o Nurolon sutures with no attempts to do a tie-over
bolster, rather utilizing just peripherally placed sutures. The
advantage of peripherally placed suture is the speed of the
bolster inset and also that it prevents the peripheral upturning
that can occur with tie-over bolsters. Postoperatively, the
sponge is kept dry and removed in 5 to 7 days. On bolster
removal and if there is any accumulation of seroma fluid, then
this is carefully expressed on the initial phase and the graft
recompressed and given a chance to continue healing
( Fig. 6.1, Fig. 6.2, Fig. 6.3, Fig. 6.4, Fig. 6.5, Fig. 6.6,
